Investigation of Kelussia odoratissima and Angelica sinensis similarities in zebrafish-based in-vivo bioactivity assays and their chemical composition

Background: Kelussia odoratissima (KO) and Angelica sinensis (AS) have been used in their indigenous traditional medicine, for various diseases. This study was conducted to evaluate the volatile oil composition of KO leaves (KVL) AS root (AVR) biological activity essential oils (EOs) hydroalcoholic extracts both plants using two different transgenic zebrafish (Danio rerio) models.Materials Methods: Both EOs were isolated by hydrodistillation analysed GC GC/MS. For viability tests, larvae treated with concentrations determine an appropriate starting concentration. Hydroalcoholic tested a dose-dependent manner tissue-specific Tg(fli-1: EGFP) Tg (ins: GFP-NTR) embryos larvae. One-way ANOVA compare mean pBC area intersegmental vessels (ISVs) outgrowth between treatment groups.Results: Eleven compounds common oils, comprising 51.3% KVL 61.7% AVR, which 39.3% 37.6% AVR phthalide structures. Results revealed that blocked ISVs formation (fli-1: increased 10% control value, while did not show any anti-angiogenesis effects these embryos. In addition, has shown significantly induce PBC regeneration following ablation GFP-NTR), but its regenerative lower than 5′-N-ethylcarboxamidoadenosine (NECA) as positive control. Taken together, could be attributed structures activity, other including β-Thujaplicinol, exclusively existing might effective.Conclusion: Although genera, organs, origin are different, similar chemical activities make them valuable resources further investigation basic medical pharmaceutical science.